Researchers from the University of Freiburg, Germany have developed a functional flow cytometry-based method for the diagnosis of innate primary immunodeficiencies (PIDs). The technique was applied to ...

Flow cytometry (FC) is a widely applied analytical technique that utilizes, typically with wavelengths of either 488, 642, 532 or 405 nanometers (nm), lasers to scan and evaluate suspended cells and ...

Human umbilical vein endothelial cells (HUVECs) were seeded at 1 × 10 4 cells per cm 2 in six-well plates. Treatment with TNF-α (2.5, 5 or 10 ng/ml) or lipopolysaccharide (0.5, 1 or 5 μg/ml) treatment ...