Light microscopy is the only way in which we can look inside a living cell, or living tissues, in three dimensions. An electron microscope only gives a two-dimensional view, and the organic sample ...

Nanoscopy describes the ability to see beyond the generally accepted optical limit of 200–300 nm. Stimulated emission depletion (STED) microscopy, developed by Stefan W. Hell and Jan Wichmann in 1994, ...

Stimulated emission depletion (STED) microscopy is one of the family of super-resolution techniques developed to allow imaging beyond the normal diffraction limit of optical microscopy. The principle ...

Researchers enhanced Stimulated Emission Depletion (STED) microscopy by replacing traditional fluorophores with nanographenes, enabling the observation of longer-duration processes, overcoming a ...

Both images show individual red blood cells. The one on the right, produced by STED, more clearly shows the Piezo1 proteins (in green), which are a mechanical calcium channel and play a crucial role ...

A new photostable fluorescent dye for super resolution microscopy could serve as a powerful tool to visualize biological events and structural details in living cells at real-time for prolonged ...

What is Structured Illumination Microscopy? Structured Illumination Microscopy (SIM) is a super-resolution fluorescence microscopy technique that allows for imaging beyond the diffraction limit of ...

A decade ago, the Nobel Prize in Chemistry was awarded to a trio of researchers for the development of super-resolved fluorescence microscopy. The announcement at the time stated that the researchers’ ...

Novel developments also focus on quick and high-speed AFM to improve temporal resolution. 2 The combination of AFM with optical microscopy has expanded the number of possible applications, ...