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The mechanism of Holliday-junction cleavage by RuvC has been investigated using artificial junctions created by annealing oligonucleotides together (Figure 5-42A). Each of the duplexes involved in these junctions has unique sequences at its ends, which allowed the oligonucleotides to anneal to form the indicated Holliday junctions; they also possess a core of 11 nucleotides that are homologous. The Holliday junction can branch migrate within the core region. A dimer of RuvC binds to Holliday jun
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